Next-Generation Sequencing in Solid Tumor Histopathology: Practical Insights and Applications

The introduction of high-throughput sequencing with Next-Generation Sequencing (NGS) marked a significant advancement in the field. Over the past decade, NGS has helped to uncover molecular alterations that drive malignant behavior and provide crucial information for clinicians, directly influencing treatment options and significantly improving patient outcomes. This book focuses on the molecular alterations and signaling pathways identifiable through NGS and on the practical challenges encountered in daily diagnostic work. These include the management of scant or degraded material, the need to correlate molecular findings with morphology and ancillary techniques, and the limited availability of advanced sequencing platforms in many routine settings.